Widely used in biochemistry and molecular biology for size exclusion chromatography (gel filtration). Effective for separating macromolecules such as proteins, nucleic acids, and polysaccharides based on molecular size. Commonly applied in protein purification workflows to remove salts, small molecules, or aggregates. Its cross-linked structure provides stability across a range of pH and denaturing conditions, making it suitable for both analytical and preparative scale separations. Also used in desalting and buffer exchange procedures due to high recovery rates of biomolecules.