Used as a calibration standard in analytical chemistry, particularly in gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). It ensures accurate identification and quantification of volatile organic compounds. Commonly applied in environmental testing to measure solvent residues, in industrial hygiene for air quality monitoring, and in pharmaceutical analysis to detect residual solvents. The n-hexane solution serves as a reliable reference for peak retention time alignment and instrument performance verification.