Validamycin A is widely used in agriculture as a fungicide, particularly effective against Rhizoctonia species that cause sheath blight in rice. It works by inhibiting fungal growth through disruption of cell wall synthesis, specifically targeting trehalase activity, which is essential for fungal development. Due to its high selectivity and low toxicity to non-target organisms, it is favored in integrated pest management programs. The compound is applied as a foliar spray or soil drench and is especially valuable in paddy fields where fungal diseases can significantly reduce crop yield. Its role in sustainable farming is enhanced by its biodegradability and minimal impact on the environment.