Used primarily in research laboratories as a reference standard or reagent for studying nitrosamines, which are known carcinogens. It serves in the analysis of environmental and biological samples to detect and quantify nitrosamine contamination, especially in tobacco products, cured meats, and certain industrial processes. Due to its instability and health hazards, it is handled under controlled conditions and not used in commercial product manufacturing. Also employed in toxicological studies to assess DNA damage and cancer mechanisms in experimental models.