Used in biochemical research as a metabolite of nicotinamide, 1-methylnicotinamide chloride plays a role in studies related to cellular metabolism and NAD+ pathways. It serves as a marker for nicotinamide metabolism in pharmacokinetic studies. It is also investigated for its potential protective effects on endothelial cells and possible anti-inflammatory properties. Due to its solubility and stability, it is commonly used in in vitro systems to modulate metabolic enzyme activity. Additionally, it has been explored in studies related to oxidative stress and mitochondrial function.