Used primarily as a reference standard in determining octane ratings for gasoline, isooctane serves as the 100-point benchmark in the octane scale due to its excellent anti-knock properties. It is blended into automotive fuels to improve combustion efficiency and reduce engine knocking. Additionally, it is employed as a non-polar solvent in laboratories for extractions and analytical procedures. Its high purity and consistent performance make it suitable for use in calibration of fuel testing equipment and in research related to fuel formulation and engine performance.