Used as a standard reference fuel in determining octane ratings for gasoline, isooctane helps measure and improve engine performance by resisting knocking and pre-ignition in internal combustion engines. Its high octane number makes it ideal for calibrating fuel blends in the petroleum industry. It is also employed as a non-polar solvent in laboratories for extractions and analytical procedures, particularly in chromatography and organic synthesis. Due to its low reactivity and consistent volatility, it serves as a carrier solvent in various chemical formulations and is used in the preparation of calibration gases for environmental and industrial testing.