Used as a pH indicator in analytical chemistry, particularly in titrations involving weak acids and bases. It exhibits a clear color change in the pH range of 3.0 to 5.0, shifting from red to yellow, making it suitable for detecting endpoint in buffer solutions and biological systems. Also employed in histology for staining tissue sections to enhance microscopic visualization of cellular structures. Its solubility in water and alcohol allows formulation in various solvent systems for industrial and laboratory applications.