Used primarily in research settings as a model compound for studying the carcinogenic effects of nitrosamines. It is employed in laboratory experiments to induce tumors in animal models, helping researchers understand the mechanisms of cancer development and test potential chemopreventive agents. Additionally, it serves as a reference standard in analytical chemistry for detecting and quantifying nitrosamine contamination in food, tobacco, and environmental samples. Its application is strictly controlled due to its high toxicity and carcinogenic properties.