Digoxigenin is widely used in molecular biology as a labeling agent for nucleic acids and proteins. It is commonly incorporated into DNA, RNA, or antibodies to enable detection in techniques like in situ hybridization, Southern blotting, and ELISA. Its high specificity and sensitivity make it a valuable tool for visualizing and quantifying target molecules in research and diagnostics. Additionally, digoxigenin-labeled probes are utilized in immunohistochemistry to study gene expression and protein localization in tissues. Its stability and compatibility with various detection systems, such as enzyme-linked or fluorescence-based methods, enhance its versatility in experimental applications.