TIN0 Broth is primarily used in microbiological testing for the cultivation and maintenance of anaerobic microorganisms. It serves as a growth medium in laboratories to support the isolation and identification of specific bacterial strains, particularly those sensitive to oxygen. The broth is useful in fermentation studies and in the preparation of inocula for antimicrobial susceptibility testing. Its composition promotes the growth of fastidious organisms by providing essential nutrients and reducing agents that maintain a low redox potential. It is commonly employed in clinical, environmental, and industrial microbiology for anaerobic culture workflows.