Thiolglycollate Medium is widely used in microbiology for the cultivation of anaerobic, microaerophilic, and aerobic microorganisms. It supports the growth of a broad range of bacteria and fungi, making it ideal for sterility testing in pharmaceutical and medical product quality control. The medium contains reducing agents that lower the oxidation-reduction potential, allowing anaerobes to grow. It is commonly used in challenge tests for detecting microbial contamination in injectable drugs, vaccines, and other sterile preparations. Its layered growth pattern helps differentiate between aerobic organisms (which grow at the top) and anaerobic organisms (which grow deeper in the tube). This makes it valuable in both clinical diagnostics and industrial microbiology for identifying contaminating organisms.