AICAR phosphate is widely utilized in biochemical research to study the AMP-activated protein kinase (AMPK) pathway, which plays a critical role in cellular energy homeostasis. It is often employed as an activator of AMPK, helping researchers understand metabolic processes such as glucose uptake, fatty acid oxidation, and mitochondrial biogenesis. This compound is also used in studies related to exercise mimetics, as it can simulate some effects of physical activity on muscle metabolism. Additionally, AICAR phosphate has been investigated for its potential therapeutic applications in conditions like diabetes, obesity, and cardiovascular diseases due to its ability to enhance energy regulation and improve metabolic function. Its role in cancer research is also notable, as it may influence tumor metabolism and growth.