Used primarily in histology and pathology laboratories to remove calcium deposits from tissue samples, enabling better sectioning and staining during microscopic examination. It softens hard tissues such as bone, calcified tumors, and atherosclerotic plaques, allowing for more accurate diagnosis. The solution is also employed in molecular biology to preserve tissue architecture while preparing samples for DNA or RNA analysis. Due to its gentle decalcification action, it minimizes damage to cellular structures and antigens, making it suitable for immunohistochemistry. Commonly used in research and clinical settings where prolonged decalcification without tissue degradation is required.