Used primarily in histology and pathology labs to remove calcium deposits from tissue samples, enabling better sectioning and staining during microscopic examination. Helps soften hard tissues such as bone, calcified tumors, or atherosclerotic plaques, making them easier to process. Commonly used in diagnostic settings to improve visualization of cellular structures. Also employed in molecular studies where DNA or RNA extraction is required from calcified tissues, as decalcification enhances nucleic acid recovery. Works gently over several days, preserving tissue morphology and antigenicity for immunohistochemistry.