D-Asparagine is the D-enantiomer of the amino acid asparagine, primarily utilized as a specialty chemical in biochemical and pharmaceutical research. It serves as a key intermediate in the synthesis of D-amino acid-containing peptides, which exhibit enhanced resistance to enzymatic degradation and have potential applications in developing novel therapeutics for neurological disorders and antimicrobial agents. In microbiological studies, it is added to culture media to investigate microbial metabolism of D-amino acids and to differentiate bacterial strains based on enantiomer specificity. Additionally, it is employed in enzymatic assays to study asparaginase activity and in chiral chromatography as a reference standard for analytical purposes.