2-Methylbutyrylcarnitine is primarily used in metabolic studies and diagnostics. It serves as a biomarker for identifying disorders related to fatty acid oxidation and organic acid metabolism. Clinically, it helps in diagnosing conditions such as short/branched-chain acyl-CoA dehydrogenase deficiency (SBCAD). Researchers analyze its levels in blood or urine to assess metabolic health and detect abnormalities in energy production pathways. Additionally, it plays a role in understanding mitochondrial function and its impact on cellular energy processes. This compound is also utilized in research to explore its connection with metabolic syndromes and potential therapeutic interventions.