Z-Phe-Leu is widely used in peptide synthesis as a protected dipeptide. It serves as a building block in the production of more complex peptides and proteins, particularly in solid-phase peptide synthesis (SPPS). The Z-group (benzyloxycarbonyl) acts as a protective group for the amine functionality, ensuring selective reactions during the synthesis process. This compound is also utilized in biochemical research to study enzyme-substrate interactions, particularly in the context of proteases and peptidases, due to its peptide bond structure. Additionally, Z-Phe-Leu is employed in the development of peptide-based drugs and inhibitors, contributing to advancements in medicinal chemistry and therapeutic applications.