Used in biochemical research to study metal ion binding properties, particularly in the context of peptides and proteins. It serves as a model compound to understand the coordination chemistry of histidine residues in metalloproteins. Applied in enzymatic studies to investigate peptide hydrolysis and enzyme specificity. Also utilized in the development of biosensors and chelating agents for metal ions, aiding in environmental and analytical chemistry. Its role in mimicking natural peptide sequences makes it valuable in drug discovery and peptide-based therapeutics.