β-Guanidinopropionic acid is primarily used in research studies related to muscle metabolism and energy regulation. It acts as a creatine analog, competitively inhibiting creatine uptake in muscle cells. This property makes it valuable for investigating the role of creatine in cellular energy processes, particularly in studies focused on muscle fatigue, endurance, and metabolic disorders. Additionally, it has been explored in preclinical research to understand its potential effects on conditions like muscular dystrophy and other neuromuscular diseases. Its ability to modulate energy metabolism also makes it a tool for studying metabolic pathways and their implications in health and disease.