Phosphocreatine di-tris salt is widely used in biochemical research and medical applications due to its role in energy metabolism. It serves as a critical component in studies focused on muscle physiology, particularly in understanding the mechanisms of ATP regeneration in muscle cells. This compound is often employed in experiments investigating cellular energy transfer, as it helps maintain ATP levels during high-energy demand activities.

In clinical settings, it is utilized in diagnostic tests to assess muscle function and energy metabolism, especially in conditions like muscular dystrophy or heart failure. Additionally, it is used in sports science to evaluate the efficiency of energy systems in athletes. Its stability and effectiveness in buffering ATP levels make it a valuable tool in both research and therapeutic contexts.