This compound is primarily used in peptide synthesis, particularly in solid-phase peptide synthesis (SPPS). It serves as a protected amino acid derivative, where the FMOC group acts as a temporary protecting group for the amino function, and the TRT group protects the imidazole side chain of histidine. This protection is crucial to prevent unwanted side reactions during the peptide chain assembly process. The N-methylation of histidine enhances the stability and bioavailability of the resulting peptides, making it valuable in the development of therapeutic peptides and peptidomimetics. Additionally, it is utilized in research to study peptide-protein interactions and enzyme mechanisms, contributing to advancements in drug discovery and biochemical studies.