DL-β-Hydroxynorvaline is primarily used in biochemical research and pharmaceutical development. It serves as a building block in the synthesis of peptides and proteins, aiding in the study of enzyme mechanisms and protein structure-function relationships. Additionally, it is utilized in the development of novel drugs, particularly those targeting metabolic disorders and neurodegenerative diseases. Its role in modulating metabolic pathways makes it valuable in exploring therapeutic interventions for conditions like diabetes and Alzheimer's disease. Furthermore, it is employed in the production of chiral compounds, which are essential in the creation of enantiomerically pure pharmaceuticals.