D-Ornithine monohydrochloride is primarily used in biochemical research and pharmaceutical applications. It serves as a crucial component in studies related to the urea cycle, where it aids in understanding metabolic processes and ammonia detoxification. Additionally, it is utilized in the production of supplements aimed at improving athletic performance and muscle recovery due to its role in arginine synthesis. In the medical field, it is investigated for its potential therapeutic effects in treating liver disorders and promoting wound healing. Its applications also extend to cell culture media, where it supports the growth and maintenance of certain cell types.