Glycyl-L-tyrosine hydrate is widely used in biochemical research as a substrate for studying enzyme activity, particularly for proteases and peptidases. It helps in understanding the mechanisms of peptide bond cleavage and enzyme kinetics. In the field of microbiology, it serves as a nutrient source in culture media to support the growth of specific microorganisms. Additionally, it is utilized in peptide synthesis and as a reference compound in chromatographic analysis to ensure accuracy and consistency in experimental results. Its role in structural studies of peptides and proteins also makes it valuable for advancing knowledge in molecular biology and biochemistry.