Used as a biochemical reagent in epigenetic research, particularly in studies involving protein methylation. It serves as a precursor or reference standard for detecting and quantifying methylated lysine residues in histone proteins. Plays a role in assays that investigate post-translational modifications linked to gene regulation, chromatin structure, and diseases like cancer. Additionally, it is used as a standard in chromatographic and mass spectrometry analyses to measure levels of naturally occurring Nε,Nε,Nε-Trimethyllysine in biological samples such as plasma or tissues. It is also employed in studies on amino acid metabolism and diseases associated with methylation disorders, such as neurological and cardiovascular diseases. Also utilized in mass spectrometry workflows for calibration and identification of methylated peptides.