Used in biochemical research for studying amino acid metabolism and enzyme mechanisms, particularly in histidine biosynthesis pathways. Serves as a precursor or analog in the synthesis of histidine-related compounds. Employed in cell culture media formulations to support the growth of histidine-requiring microbial strains. Also utilized in pharmaceutical research for developing enzyme inhibitors and metabolic probes. Its stability and solubility make it suitable for in vitro studies involving amino acid transport and regulation.