Used extensively in enzymatic assays, particularly for studying protease activity. The compound acts as a fluorogenic substrate, releasing a fluorescent signal upon cleavage by specific enzymes such as glutamate carboxypeptidases. This allows real-time monitoring of enzyme kinetics and inhibition in research and drug development. Commonly employed in high-throughput screening and biochemical assays due to its sensitivity and specificity. Also utilized in cellular and molecular biology to investigate enzyme function in lysates and live-cell systems.