DL-Glutamine is widely used in cell culture applications, particularly in the growth of mammalian cells. It serves as a crucial source of nitrogen and carbon, supporting protein synthesis and energy production within cells. In biopharmaceutical manufacturing, it is added to culture media to enhance the yield and viability of monoclonal antibodies and recombinant proteins. Due to its role in cellular metabolism, it is also utilized in research settings studying cancer cell proliferation, immune function, and neurological processes. In some cases, it is employed in microbial fermentation processes where glutamine metabolism is part of the pathway under investigation. Its stability in solution makes it suitable for use in both batch and fed-batch culture systems.