Used in biochemical research as a metabolite analog to study metabolic pathways, particularly in relation to mitochondrial function and Krebs cycle regulation. It serves as a tool compound in investigations of hydroxyglutarate dehydrogenase activity and related enzymatic processes. Also employed in cell culture media formulations to examine the effects of accumulated organic acids on cellular metabolism. Its disodium salt form enhances solubility and stability in aqueous solutions, making it suitable for in vitro studies. Investigated in models of metabolic disorders associated with D-2-hydroxyglutaric aciduria to understand disease mechanisms and potential therapeutic interventions.