Dansylglycine is primarily used as a fluorescent probe in biochemical and biophysical research. Its strong fluorescence properties make it valuable for studying protein interactions, conformational changes, and binding sites. It is often employed in fluorescence spectroscopy and microscopy to label proteins or peptides, enabling visualization and analysis of molecular dynamics. Additionally, dansylglycine is utilized in enzyme studies to investigate substrate specificity and catalytic mechanisms. Its ability to act as a tracer in cellular and molecular systems also aids in understanding biological processes at the molecular level.