His-Leu, a dipeptide composed of histidine and leucine, is primarily utilized in the field of biomedical research and pharmaceutical development. It serves as a model compound for studying peptide interactions and enzymatic processes, particularly in the context of proteolysis and peptide bond cleavage. In nutritional science, His-Leu is explored for its potential role in enhancing protein synthesis and muscle recovery, making it relevant in sports nutrition and dietary supplements. Additionally, it is investigated for its bioactive properties, which may contribute to its use in developing therapeutic agents for metabolic and neurological disorders. Its stability and bioavailability also make it a candidate for drug delivery systems and peptide-based therapies.