N-Acetyl-DL-tryptophan is primarily used in the field of biochemistry and medical research. It serves as a precursor in the synthesis of various biologically active compounds, particularly in studies related to neurotransmitter regulation and brain function. This chemical is often utilized in experimental models to investigate its potential effects on mood disorders, such as depression and anxiety, due to its role in serotonin production. Additionally, it is employed in the development of pharmaceutical formulations aimed at enhancing cognitive function and mental health. In some cases, it is also used as a stabilizer or additive in protein-based therapies to improve their efficacy and stability.