N-Acetyl-DL-aspartic acid is primarily used in neuroscience research as a biochemical tool to study the role of N-acetylaspartate (NAA) in the brain. It serves as a precursor or analog in experiments investigating NAA's involvement in neuronal metabolism and osmoregulation. In medical research, it is utilized to explore its potential in diagnosing and understanding neurological disorders such as Canavan disease, multiple sclerosis, and brain tumors, where NAA levels are often altered. Additionally, it is employed in the development of imaging techniques like magnetic resonance spectroscopy (MRS) to monitor brain health and disease progression.