N-Acetyl-D-alanine is primarily used in biochemical research and pharmaceutical applications. It serves as a chiral building block in the synthesis of peptides and other complex organic molecules, particularly in the development of drugs and bioactive compounds. Its role in peptide synthesis is crucial for creating specific amino acid sequences that mimic natural proteins or enzymes. Additionally, it is utilized in studies related to enzyme mechanisms and metabolic pathways, helping researchers understand biological processes at a molecular level. In the pharmaceutical industry, it contributes to the production of enantiomerically pure drugs, ensuring higher efficacy and reduced side effects.