L-Norleucine is widely used in biochemical research as a non-natural amino acid to study protein structure and function. It serves as a valuable tool in metabolic pathway analysis, helping researchers understand amino acid metabolism and enzyme specificity. In pharmaceuticals, it is utilized in the development of peptide-based drugs, where it can enhance stability and bioavailability. Additionally, L-Norleucine is employed in nutritional studies to investigate its role as a dietary supplement, particularly in muscle growth and recovery. Its incorporation into microbial fermentation processes also aids in the production of various bioactive compounds.