L-Aspartic acid 4-tert-butyl ester is widely used in organic synthesis as a building block for peptides and other biologically active compounds. It serves as a key intermediate in the production of pharmaceuticals, particularly in the development of drugs targeting neurological disorders and metabolic diseases. The tert-butyl ester group provides protection for the carboxylic acid functionality during synthesis, allowing for selective reactions to occur. This compound is also utilized in the preparation of chiral catalysts and ligands, which are essential in asymmetric synthesis for creating enantiomerically pure substances. Additionally, it finds applications in research and development for studying enzyme mechanisms and protein interactions due to its structural similarity to natural amino acids.