(S)-SNAP5114 is primarily used in research settings as a selective inhibitor of the GABA transporter 1 (GAT1). Its application is significant in neuroscience studies, particularly in understanding the role of GABAergic neurotransmission in the central nervous system. Researchers utilize (S)-SNAP5114 to investigate conditions such as epilepsy, anxiety, and other neurological disorders where GABA signaling is implicated. By inhibiting GAT1, it helps in studying the modulation of GABA levels, which can provide insights into potential therapeutic strategies for these disorders. Additionally, it is employed in electrophysiological experiments to observe the effects of altered GABA uptake on neuronal activity.