Chymotrypsin is widely used in medical and biotechnological applications due to its proteolytic activity. In medicine, it is employed as an anti-inflammatory agent to reduce swelling and promote healing in conditions like postoperative wounds, ulcers, and traumatic injuries. It helps in breaking down damaged tissue and clearing debris, accelerating tissue repair.

In the field of biochemistry, chymotrypsin is utilized for protein digestion and peptide mapping, aiding in the study of protein structure and function. It is also used in cell culture techniques to dissociate tissues into individual cells. Additionally, chymotrypsin finds application in the food industry for protein hydrolysis, improving the texture and flavor of certain products. Its specificity for cleaving peptide bonds makes it a valuable tool in various research and industrial processes.