DL-Homocysteine is primarily used in biochemical research to study metabolic pathways, particularly those involving sulfur-containing amino acids. It serves as a key intermediate in the methionine cycle and is often utilized to investigate disorders related to homocysteine metabolism, such as hyperhomocysteinemia. Researchers employ it to understand the impact of elevated homocysteine levels on cardiovascular health, neurological function, and bone density. Additionally, it is used in the development and testing of diagnostic assays to measure homocysteine concentrations in blood or plasma, aiding in the diagnosis of conditions like vitamin B12 or folate deficiency. In pharmacology, DL-Homocysteine is used to explore potential therapeutic interventions targeting homocysteine-related diseases. Its role in oxidative stress and endothelial dysfunction also makes it a valuable tool in studying the mechanisms of atherosclerosis and other vascular diseases.