L-Hydroxyproline is widely used in the pharmaceutical and cosmetic industries due to its role in collagen synthesis. It supports skin health by promoting collagen stability, making it a key ingredient in anti-aging skincare products. It is also used in wound healing formulations and tissue engineering because of its involvement in extracellular matrix formation. In medicine, it serves as a biomarker for collagen turnover in conditions like osteoporosis, fibrosis, and connective tissue disorders. Additionally, it is included in cell culture media to support the growth of mammalian cells requiring collagenous matrices.