L-Proline is widely used in the pharmaceutical industry as a chiral building block for synthesizing drugs, especially those targeting cardiovascular and central nervous system disorders. It plays a key role in peptide synthesis, improving stability and bioavailability of therapeutic peptides.

In cell culture and biotechnology, L-Proline supports the growth of mammalian cells and is involved in the production of recombinant proteins. It serves as an osmoprotectant in bioprocessing, protecting cells from stress during fermentation and large-scale protein production.

It is also used in skincare formulations due to its role in collagen synthesis, helping improve skin elasticity and repair.

Additionally, L-Proline is utilized in animal feed and nutritional supplements to support joint and muscle health, as it is involved in connective tissue formation.