L-Valine is widely used in the pharmaceutical industry for the formulation of amino acid infusions, particularly in parenteral nutrition to support patients who cannot obtain sufficient nutrients orally. It plays a key role in muscle metabolism and is included in supplements for athletes and individuals recovering from surgery or illness to promote tissue repair and prevent muscle wasting.

In biotechnology, L-Valine serves as a component in cell culture media, supporting the growth of mammalian cells during the production of vaccines and recombinant proteins. It is also used in research settings to study metabolic pathways and amino acid transport systems.

In agriculture, L-Valine is added to animal feed to improve growth performance and feed efficiency, especially in poultry and swine. Its inclusion helps meet essential amino acid requirements, supporting overall health and development.

Additionally, L-Valine is used in the synthesis of certain drugs and bioactive peptides, where it contributes to the structural and functional properties of the molecule. It is also employed in the cosmetics industry to nourish the skin and enhance collagen production.