DL-Norvaline is used in biochemical research as an analog of natural amino acids to study protein synthesis and enzyme activity. It serves as an intermediate in the development of pharmaceuticals, particularly in the design of enzyme inhibitors. The compound is also utilized in cell culture media to support the growth of certain microorganisms and in studies related to metabolic pathways. Due to its structural similarity to valine, it can be incorporated into peptides to modify their biological activity or stability. Additionally, DL-Norvaline finds use in the production of specialty chemicals and as a reagent in chiral synthesis.