Used in tissue dissociation to break down proteins and separate cells in cell culture applications. Commonly employed in enzymatic digestion for preparing single-cell suspensions from tissues. Utilized in protein sequencing and peptide mapping due to its specificity for cleaving peptide bonds at aromatic amino acids. Applied in eye surgery, particularly in cataract procedures, to weaken zonular fibers. Also used in some digestive enzyme supplements and in research for studying protease activity and enzyme kinetics.