Widely used in cell culture laboratories to dissociate cells from the surface of culture dishes by breaking down adhesion proteins. Commonly applied in trypsin-EDTA-free cell detachment protocols where milder proteolytic activity is needed. Used in protein digestion workflows for mass spectrometry analysis, particularly when targeting cleavage at aromatic amino acids like tyrosine, tryptophan, and phenylalanine. Also utilized in enzymatic debridement of burn wounds to remove dead tissue, promoting healing in clinical settings. Its specificity makes it valuable in peptide mapping and structural studies of proteins.