Glycine hydrochloride is widely used in biochemical research as a buffering agent to maintain stable pH levels in various experimental setups. It is particularly useful in electrophoresis and chromatography techniques where precise pH control is essential for accurate results.

In the pharmaceutical industry, it serves as an intermediate in the synthesis of more complex compounds, including certain drugs and active pharmaceutical ingredients. Its role in stabilizing formulations and enhancing the solubility of other substances makes it valuable in drug development.

Additionally, glycine hydrochloride is employed in the food industry as a flavor enhancer and preservative. It helps improve the taste of certain products while extending their shelf life by inhibiting microbial growth.

In industrial applications, it is used in metal surface treatment processes, where it aids in cleaning and preparing metals for coating or plating, ensuring better adhesion and durability of the final product.