N8-Acetylspermidine dihydrochloride is primarily utilized in biochemical research to study polyamine metabolism and its role in cellular processes. It serves as a valuable tool in investigating the functions of acetylation in spermidine, which is crucial for understanding cell growth, proliferation, and differentiation. Researchers use it to explore its potential impact on autophagy, a cellular recycling process linked to aging and disease. Additionally, it is employed in studies related to cancer biology, as polyamines are often upregulated in tumor cells. Its application extends to neuroscience, where it helps examine the role of polyamines in neuronal signaling and brain function. This compound is also used in developing assays to measure polyamine levels in biological samples, aiding in diagnostic and therapeutic research.