L-4-Oxalysine hydrochloride is primarily used in biochemical research to study amino acid metabolism and enzyme inhibition. It acts as an antagonist of lysine, making it valuable in experiments exploring the role of lysine in biological systems. Researchers utilize it to investigate cellular processes, particularly those involving lysine-dependent enzymes and pathways. Additionally, it has been employed in studies related to cancer research, where its ability to interfere with lysine metabolism helps in understanding tumor growth and potential therapeutic targets. Its application extends to the development of novel inhibitors for enzymes that rely on lysine, contributing to advancements in drug discovery and metabolic studies.