Calcium 3-methyl-2-oxobutyrate is utilized in the production of dietary supplements to enhance athletic performance and aid muscle recovery. It functions in key metabolic pathways, specifically through transamination to produce valine, a branched-chain amino acid essential for muscle protein synthesis and repair. This compound is also applied in research investigating metabolic disorders, such as branched-chain amino acid metabolism defects, and in the development of therapeutic interventions. In the pharmaceutical sector, it is incorporated into formulations to support metabolic health and treat conditions associated with amino acid deficiencies, particularly those involving valine.